Stress response methods and protocols / edited by Stephen M. Keyse

Online: Connect to OhioLINK EBC
Location:
Retrieving Holdings Information
Subjects: Stress (Physiology)--Laboratory manuals
Molecular biology--Laboratory manuals
Cellular signal transduction--Laboratory manuals
Heat shock proteins--Laboratory manuals
Gene expression--Laboratory manuals
Formats: Electronic Resource, Remote
Material Type: Books
Language: English
Audience: Unspecified
Published: Totowa, NJ : Humana Press, c2000
Series: Methods in molecular biology v. 99
Methods in molecular biology (Clifton, N.J.) v. 99
Table of Contents: Preface
Contributors
Pt. I. Detection and Assay of Stress-Induced Damage
1. Identifying and Counting Protein Modifications Triggered by Nitrosative Stress / Prabodh K. Sehajpal, Harry M. Lander 3
2. Determination of Carbonyl Groups in Oxidized Proteins / Rodney L. Levine, Nancy Wehr, Joy A. Williams 15
3. Quantitation of 4-Hydroxynonenal Protein Adducts / Koji Uchida, Earl R. Stadtman 25
4. Detection of Oxidative Stress in Lymphocytes Using Dichlorodihydrofluorescein Diacetate / Cecile M. Krejsa, Gary L. Schieven 35
5. The Measurement of Protein Degradation in Response to Oxidative Stress / Thomas Reinheckel, Tilman Grune, Kelvin J. A. Davies 49
Pt. II. The Activation of Signal Transduction by Cellular Stress
6. Analysis of the Role of the AMP-Activated Protein Kinase in the Response to Cellular Stress / D. Grahame Hardie, Ian P. Salt, Stephen P. Davies 63
7. Detection and Activation of Stress-Responsive Tyrosine Kinases / Gary L. Schieven 75
8. Detection of DNA-Dependent Protein Kinase in Extracts from Human and Rodent Cells / Yamini Achari, Susan P. Lees-Miller 85
9. Expression and Assay of Recombinant ATM / Yael Ziv, Sharon Banin, Dae-Sik Lim 99
10. Detection and Purification of a Multiprotein Kinase Complex from Mammalian Cells: IKK Signalsome / Frank Mercurio, David B. Young, Anthony M. Manning 109
11. Methods to Assay Stress-Activated Protein Kinases / Ana Cuenda 127
12. Monitoring the Activation of Stress-Activated Protein Kinases Using GAL4 Fusion Transactivators / Chao-Feng Zheng, Li Xu 145
13. Use of Kinase Inhibitors to Dissect Signaling Pathways / Ana Cuenda, Dario R. Alessi 161
14. The Development and Use of Phospho-Specific Antibodies to Study Protein Phosphorylation / Jeremy P. Blaydes, Borek Vojtesek, Graham B. Bloomberg 177
15. Peptide Assay of Protein Kinases and Use of Variant Peptides to Determine Recognition Motifs / D. Grahame Hardie 191
Pt. III. The Analysis of Stress-Induced Gene Expression
16. Assaying NF-[kappa]B and AP-1 DNA-Binding and Transcriptional Activity / Judith M. Mueller, Heike L. Pahl 205
17. Analysis of the Mammalian Heat Shock Response: Inducible Gene Expression and Heat Shock Factor Activity / Anu Mathew, Yanhong Shi, Caroline Jolly 217
18. Approaches to Define the Involvement of Reactive Oxygen Species and Iron in Ultraviolet-A Inducible Gene Expression / Charareh Pourzand, Olivier Reelfs, Rex M. Tyrrell 257
19. The Human Immunodeficiency Virus LTR-Promoter Region as a Reporter of Stress-Induced Gene Expression / Michael W. Bate, Sushma R. Jassal, David W. Brighty 277
20. SAGE: The Serial Analysis of Gene Expression / Jill Powell 297
21. Analysis of Differential Gene Expression Using the SABRE Enrichment Protocol / Daniel J. Lavery, Phillippe Fonjallaz, Fabienne Fleury-Olela 321
22. UVB-Regulated Gene Expression in Human Keratinocytes: Analysis by Differential Display / Harry Frank Abts, Thomas Welss, Kai Breuhahn 347
Pt. IV. Analysis of Stress Protein Function
23. Heme Oxygenase Activity: Current Methods and Applications / Stefan W. Ryter, Egil Kvam, Rex M. Tyrrell 369
24. Analysis of Molecular Chaperone Activities Using In Vitro and In Vivo Approaches / Brian C. Freeman, Annamieke Michels, Jaewhan Song 393
25. Analysis of Chaperone Properties of Small Hsp's / Monika Ehrnsperger, Matthias Gaestel, Johannes Buchner 421
26. Analysis of Small Hsp Phosphorylation / Rainer Benndorf, Katrin Engel, Matthias Gaestel 431
27. Analysis of Multisite Phosphorylation of the p53 Tumor-Suppressor Protein by Tryptic Phosphopeptide Mapping / David W. Meek, Diane M. Milne 447
28. The Development of Physiological Models to Study Stress Protein Responses / Ted R. Hupp 465
Index 485
Alternate Titles: In: OhioLINK electronic book center (OCoLC)180989150
In: SpringerLink (OCoLC)43927870
Issued in other form: Stress response : methods and protocols (OCoLC)42475447
Additional Authors: Keyse, Stephen M
Notes: LCCN: 99042177
ISBN: 0896036111 (alk. paper)
ISBN: 9780896036116 (alk. paper)
Includes bibliographical references and index
Partial contents: pt. 1. Detection and assay of stress-induced damage -- Identifying and counting protein modificatins triggered by nitrosative stress -- Determinatin of carbonyl groups in oxidized proteins -- Quantitation of 4-hydroxynonenal protein adducts -- Detection of oxidative stress in lymphocytes using dichlorodihydrofluorescein diacetate -- The measurement of protein degradation in response to oxidative stress -- pt. 2. Teh activation of signal transduction by cellular stress -- Analysis of the role of teh AMP-activated protein kinase in the response to cellular stress -- Detection and activation of stress-responsive tyrosine kinases -- Detection of DNA-dependent protein kinase in extracts from human and rodent cells -- pt. 3. The analysis of stress-induced gene expression -- pt. 4. Analysis of stress protein function
Available to OhioLINK libraries
Reproduction notes: Electronic resource. Berlin : Springer, 2008. s2008 gw n s
Mode of access: World Wide Web
Physical Description: xvi, 488 p. : ill. ; 24 cm
OCLC Number: 261067135
ISBN/ISSN: 0896036111
9780896036116

Miami University Libraries | 151 South Campus Avenue | Oxford, Ohio 45056 | 513.529.4141 | Miami University | myMiami | Staff Area